Sartorius, 414.1218-2, CIMmultus® Oligo dT18 (C6 Linker) 4 mL Monolithic Column (2 μm)

This high-performance column is primarily intended for capturing ssRNA with a poly-adenylated tail, making it an excellent tool for the purification of mRNA in IVT reaction mixtures.

CIMmultus® Monoliths are reusable chromatography devices for scalable high-resolution purification of complex biological samples. Inside the custom designed housing is a single piece stationary phase with homogeneous channel size and surface chemistry. This stationary phase is designed to simplify method transfer from development into production.
They can be used as single-use disposable or multi-use columns, and they are available in various volume ranges – from 1mL for screening and method development purposes up to 40L for industrial manufacturing.
The ligand in CIMmultus® Oligo dT18 is an 18-mer deoxythymidine chain covalently immobilised on the monolith with a carbon linker. RNA with a poly(A) tail binds on the ligand by hybridisation between adenine (A) bases on the RNA tail and deoxythymine (T) bases of the ligand. Species that do not contain a poly(A) tail do not bind on the column and are found in the unbound (flow-through) fraction.
Oligo dT (deoxythymine) features:
Oligo dT ligands covalently bound to a solid support
Hybridises to the poly-adenylated tail which is present on most eukaryotic mRNAs, or synthetised onto the molecule during IVT
Primarily intended for fast and efficient capture and purification of mRNA with a poly-adenylated tail, from various sources
Can selectively remove impurities from the sample
Convenient approach for initial purification of mRNA
Benefits:
Fewer unit operations: apply IVT material to monolith without buffer exchange
Very fast processes by convective mass transport – resolution independent of flow
Ready to use columns
High-capacity for large molecules due to fully accessible void volume in the channels
Laminar flow minimizes shear stress resulting in high yields

Specifications
Applications:
Application: Preparative Applications

Compliance Information:
GMP Compliance: non-cGMP

Connections (Physical):
Connector(s): 10-32 UNF coned port, 0.0625" OD tubing connection

Dimensions:
Channel Size: 2 µm
Column Tube Length: 28 mm
Inner Diameter: 6.5 mm
Outer Diameter: 15 mm

Features:
Reusable: Yes

General Specifications:
Flow Rate: 0.4–50 mL/min | 2.4–335 cm/h | 0.1–12.5 CV/min

Materials of Construction:
Materials of Construction: Poly(glycidyl methacrylate -co- ethylene dimethacrylate)

Operating Conditions:
Maximum Operating Pressure: 2.0 MPa | 20 bar | 290 psi
Recommended Operating Temperature: 4 °C (39 °F) to 30 °C (86 °F)
pH Stability: Linkage stability: pH 2–13. Ligand stability not tested. Functionality retained after exposure to 0.1 M NaOH for a few hours.

Physicochemical Information:
Bed Volume: 4 mL
Ligand: Oligo dT18 coupled to CDI-activated matrix, C6 Linker
Ligand Density: > 0.5 mg Oligo dT/mL wet support

Product Information:
Brand: CIMmultus®
Package Type: Individually Packed

Volume:
Volume: 4 mL