Thermo Fisher, 10103D, Dynabeads™ His-Tag Isolation and Pulldown

2 mL Dynabeads™ His-Tag Isolation and Pulldown beads are 1-μm monosized, monodispersed, superparamagnetic beads with an NTA-Cobalt-based ligand which has a high affinity towards His-tags and similar structures on molecular targets. Their small but uniform bead size presents a high surface area per mg beads and a correspondingly high capacity for the target molecule. They enable single-step isolation of tagged molecules in manual or automated workflows that helps ensure good recovery of the target. Dynabeads His-Tag Isolation and Pulldown beads can also be a great starting point for other advanced experimental approaches where solid magnetic support is needed. Benefits of Dynabeads His-Tag and Pulldown beads include:
•Performance—high sensitivity and reproducibility securing consistent results
•Efficiency—fast binding kinetics and high binding capacity
•Flexibility—flexible protocols with gentle and efficient liquid-phase reaction kinetics
•Automation-ready—automation protocols available with the KingFisher Purification instrumentsGood signal-to-noise for numerous applicationsDynabeads His-Tag Isolation and Pulldown beads are an ideal tool for isolation of tagged recombinant proteins. They are also suited for other applications with high signal-to-noise requirements. Using the principles of immobilized metal affinity binding together with the inert surface of Dynabeads magnetic beads helps ensure a clean capture with extremely low carryover of unwanted proteins. Since the bead surface is loaded with cobalt ions, it is also possible to make a stable and lasting bond with the target molecule through oxidation if the downstream applications require it. The beads are added to the solution containing the target and mixed for 5-10 minutes. After washing, the target is eluted off and ready for downstream applications. More than just a pulldownIn addition to being a great tool for protein pulldown and isolation, the Dynabeads His-Tag Isolation and Pulldown beads can serve as a platform for target immobilization. The bead carries cobalt Co2+ ions, so it is possible to cement the bond between the bead and the target just by using mildly oxidative conditions, such as 10 mM hydrogen peroxide for 30–60 minutes. This oxidizes cobalt to the Co3+ state, making the connection between the bead and the target permanent. It will not elute in the commonly used elution buffers for IMAC. This can later be used for applications requiring a strongly bound target, without the risk of reduced target yield. Automation-ready for use with KingFisher purification systemsThe 1-μm magnetic beads are ideal for high-throughput enrichment. This process can be automated using any of ourKingFisher sample purification systemsor liquid handlers. Commercial supplyOur manufacturing sites are ISO 13485-certified. Bead characteristics and manufacturing conditions help ensure consistency and batch reproducibility making them an ideal choice for commercial supply. If you are in the process of customizing Dynabeads His-Tag Isolation and Pulldown beads in a commercial product or service or need a larger bulk volume of this product, please contact us atoemdynal@lifetech. comor see ourDynabeads OEM page. Learn more about Dynabeads magnetic beads and KingFisher Sample Purification Systems:
•See our immunoprecipitation selection table, data, and references
•Dynabeads magnetic beads selection guide
•See magnetsfor Dynabeads magnetic beads separations
•KingFisher automation protocols
•Watch a video about the KingFisher Flex instrumentBinding Property: 1.6 mg of a 28 kDa histidine-tagged protein/mL (40 mg) beads
Certifications/Compliance: ISO9001 and ISO13485
Color: Brown
Concentration: 40 mg/mL
Diameter (Metric): 1 μm
Final Product Type: His-Tagged Protein
For Use With (Application): Isolation of His-tagged Recombinant Proteins
For Use With (Equipment): KingFisher Sample Purification System, DynaMag magnets
Format: Magnetic beads
High-throughput Compatibility: Yes
Ligand Type: Nitrilotriacetic acid (NTA)
Material: Magnetic Polystyrene
Quantity: 2 mL
Regulatory Status: For Research Use Only
Sample Type: Cell Cultures, Tissue Digests, Intact Proteins, Cell Lysates, Cell Extracts
Shelf Life: 36 months from date of manufacture
Shipping Condition: Ambient temperature
Sufficient For: 40 Tests
Surface Functionality: Cobalt-based immobilized metal affinity chromatography (IMAC)
Target: His-tagged proteins
Product Line: Dynabeads™
Type: Magnetic Beads
Unit Size: Each