Thermo Fisher, 11754100, CellsDirect™ One-Step qRT-PCR Kit with ROX, for ABI 7000, 7300, 7700, and 7900(HT) instruments

100 Reactions The CellsDirect™ One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.
Features of the CellsDirect™ One-Step qRT-PCR Kit include:
•Efficiency—Offers significant time and cost savings for increased throughput
•Convenience—Enzyme mix includes both SuperScript™ III Reverse Transcriptase and Platinum™TaqDNA Polymerase
•Sensitivity—No separate RNA isolation step increases sensitivity by minimizing sample loss
•Broad Detection Range—Detect targets from samples ranging from a single cell to 10,000 cells
•Versatility—Compatible with a wide variety of cell lines and samples collected by Laser Capture Microdissection (LCM)Streamline Your WorkflowIn traditional real-time quantitative RT-PCR (qRT-PCR), you first isolate RNA from cells in a time-consuming procedure that can lead to loss of material. Using the CellsDirect™ One-Step qRT-PCR Kit, you lyse the cells and add the complete lysate directly to a one-step qRT-PCR reaction with minimal handling and sample loss.
Ideal for Your qRT-PCR ApplicationCellsDirect™ technology is validated for gene expression studies involving limited samples, and is suitable for validation of RNAi knockdowns. CellsDirect™ One-Step qRT-PCR Kits are especially valuable for high-throughput applications by eliminating costly, time-consuming RNA purification steps (Figure 1). The CellsDirect™ One-Step qRT-PCR Kit may also be used to detect genomic DNA targets.
CellsDirect™ One-Step qRT-PCR Kits may be used in conjunction with hydrolysis probes (e.
g. TaqMan™) or LUX™ fluorogenic primers.
Note:This kit includes 2X Reaction Mix containing ROX™ Reference Dye. An equivalent kit (11753-500), containing a separate tube of ROX™ Reference Dye, is also available.
Concentration: 2X
For Use With (Equipment): 7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf™ MasterCycler™ RealPlex, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system
No. of Reactions: 100 Reactions
Product Line: CellsDirect™, Platinum™, SuperScript™
Quantity: 100 reactions
Sample Type: Cells
Detection Method: Primer-probe
For Use With (Application): RT-PCR
Reaction Speed: Fast or Standard
Unit Size: Each