Thermo Fisher, 11755250, SuperScript™ VILO™ Master Mix

250 Reactions SuperScript VILO Master Mix is a first-strand cDNA synthesis master mix for two-step RT-qPCR applications. The master mix combines SuperScript III Reverse Transcriptase (RT) and other RT reaction components in a single-tube format to enable reliable first-strand synthesis and high cDNA yields from a wide range of input RNA concentrations. The high RT efficiency of SuperScript III RT and the optimized formulation allow the master mix to deliver high linearity in cDNA synthesis across a broad range of input RNA template for two-step quantitative RT-PCR assays.
Features of SuperScript VILO Master Mix include:
• Starting material can range up to 2.
5 μg of total RNA in a 20 μL cDNA synthesis reaction
• Recombinant ribonuclease inhibitor safeguards against the degradation of target RNA due to ribonuclease contamination
• RT is engineered with reduced RNase H activity and increased thermal stabilityFor superior cDNA synthesis performance in RT-qPCR applicationsSuperScript IV VILO Master Mixelevates the trusted VILO technology to the next level with the highly processive and thermostable SuperScript IV RT and further optimized buffer. The master mix enables efficient cDNA synthesis at higher temperatures in less time and provides superior cDNA yield and sensitivity even with suboptimal purity or scarce templates.
Concentration: 5X
Final Product Type: First-Strand cDNA
Format: Master Mix
No. of Reactions: 250 Reactions
Optimal Reaction Temperature: 42°C
Quantity: 250 reactions
Reaction Format: Master Mix
Reagent Type: Reverse Transcription
Reverse Transcriptase: SuperScript III
Ribonuclease H Activity: Reduced
Shipping Condition: Dry Ice
Size (Final Product): Up to 10 kb
Starting Material: RNA
Technique: Reverse Transcription
Detection Method: qPCR
For Use With (Application): Real Time PCR (qPCR)
GC-Rich PCR Performance: High
Reaction Speed: 60 min.
Unit Size: Each