Thermo Fisher, 11900073, MEM α, nucleosides, powder

10 L Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of Gibco™ MEM α modifications for a range of cell culture applications. Find the right formulation using themedia selector tool.
This MEM α is modified as follows:WithWithout
• Ribonucleosides
• Sodium Bicarbonate
• Deoxyribonucleosides
• L-glutamine
• Phenol RedThe completeformulationis available.
Gibco™ MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells. This product is made with Earle’s salts.
Product Intended UseForin vitrodiagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10%Fetal Bovine Serum(FBS). MEM α uses a sodium bicarbonate buffer system (2.
2 g/L) and therefore requires a 5-10% CO2environment to maintain physiological pH. Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (seeprotocolfor details).
Cell Line: HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Cell Type: Primary Rat Astrocytes
Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
Product Line: Gibco™
Product Type: MEM α (Minimum Essential Medium α)
Quantity: 10 L
Shelf Life: 36 Months From Date of Manufacture
Shipping Condition: Room Temperature
Classification: Animal Origin-free
Form: Powder
Serum Level: Standard Serum Supplementation
Sterility: Sterile-filtered
With Additives: Low Glucose, Glutamine, Phenol Red, Sodium Pyruvate, Ribonucleosides, Deoxyribonucleosides
Without Additives: No HEPES, No Sodium Bicarbonate
Unit Size: Each