Thermo Fisher, 12390035, Ham's F-10 Nutrient Mix, HEPES

Quantity: 500 mL Ham's F-10 Nutrient Mixture (F-10) was designed for serum-free growth of Chinese Hamster Ovary (CHO) cells. F-10 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including COS-7, primary rat astrocytes, and rat prostate epithelial cells.
This Ham's F-10 Nutrient Mixture is modified as follows:With
• Phenol Red
• HEPES
• L-glutamineThe completeformulationis available.
Compared to other basal media, F-10 contains a wider variety of components, including zinc, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-10 has led to a variety of improved formulations, such as Ham's F-12 Nutrient Mixture (F-12).
Using Ham's F-10 Nutrient MixtureGibco™ F-10 contains no proteins or growth factors. Therefore, F-10 requires supplementation, commonly with 10%Fetal Bovine Serum(FBS). F-10 uses a sodium bicarbonate buffer system (1.
2 g/L) and therefore requires a 5-10% CO2environment to maintain physiological pH.
Cell Line: CHO, COS-7, and rat prostate epithelial cells
Cell Type: Primary Rat Astrocytes
Concentration: 1 X
Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
Product Line: Gibco™
Product Type: Ham's F-10 Nutrient Mixture
Quantity: 500 mL
Shelf Life: 12 Months From Date of Manufacture
Shipping Condition: Room Temperature
Classification: Animal Origin-free
Form: Liquid
Sterility: Sterile-filtered
With Additives: High Glucose, Glutamine, HEPES, Phenol Red, Sodium Pyruvate
Unit Size: Each