Thermo Fisher, 12659017, Sf9 cells in Sf-900™ III SFM

Sf9 cells in Sf-900™ III SFM Gibco™ Sf9 cells are commonly used to isolate and propagate recombinant baculoviral stocks and to produce recombinant proteins. Gibco™ Sf9 cells are adapted to serum-free suspension culture in Gibco™ Sf-900™ III SFM, which saves significant time and expense associated with the adaptation of cultures. Gibco™ Sf9 cells (frozen in Gibco™ Sf-900™ III SFM) feature:
• Recombinant protein expression from a variety of expression systems
• Good growth in adherent or suspension culture
• Small, regular size that generates even monolayers and plaques
• Documented lineage from a low passage Master Cell Bank
• Quality and performance testingRecombinant protein expression from a variety of expression systemsHigh levels of protein expression in Sf9 cells can be obtained using either theBaculoDirect™ Baculovirus Expression System, theBac-to-Bac™ Baculovirus Expression System, or theInsectDirect™ Expression System.
Good growth in adherent or suspension cultureEach vial containing 1 × 107cells can be thawed directly into suspension or adherent culture. Protocols for adherent and suspension growth in Gibco™Sf-900™ III SFMare available in the product manual. Cultures can easily be transferred between the two conditions for ease of workflow but we recommend the stock cultures are maintained in suspension.
Small, regular size that generates even monolayers and plaquesGibco™ Sf9 cells generate a clean, even monolayer and plaques due to their small, round, regular size. Other cells often form more irregular monolayers and plaques.
Documented lineage from a low passage Master Cell BankGibco™ Sf9 cells (in Sf-900™ III SFM) were prepared as serum-free, suspension cultures from Sf9 cells that originated at the USDA Insect Pathology Laboratory. The original Sf9 cells were cloned from the parental IPLBSF-21 (Sf21) cell line that was derived from the pupal ovarian tissue of the fall army worm,Spodoptera frugiperda. The serum-free Master Cell Banks were prepared at passage 26.
Quality and performance testingEach lot of Gibco™ Sf9 cells is tested for cell growth and viability post-recovery from cryopreservation. In addition, the Master Seed Bank has been tested for contamination of bacteria, yeast, mycoplasma and virus and has been characterized by isozyme and karyotype analysis.
Product UseFor Research Use Only. Not for any animal or human therapeutic or diagnostic use. Caution: Handle as potentially biohazardous material under at least Biosafety Level 2 containment. This product contains Dimethyl Sulfoxide (DMSO), a hazardous material. Review the Material Safety Data Sheet before handling.
Media Recommendation: Sf-900 III SFM (Serum-Free Media)
Product Line: Sf-900™
Product Type: Insect Cells
Quantity: 1 Vial
Shipping Condition: Dry Ice
Cell Line: Sf9
Cell Type: Insect Cells
Species: S. frugiperda
Unit Size: Each