Thermo Fisher, 12780052, LB Broth Base (Lennox) Invitrogen™

LB Broth Base (Lennox) Invitrogen™ LB Broth Base (Lennox formulation) is a popular, rich medium used to cultivate bacteria and perform coliphage plaque assays. The original formulation was published in 1951 by Guiseppe Bertani in his study of phage plaque formation and named Lysogeny broth. It is also known as Lennox broth and Luria-Bertani broth.
Widely used LB medium formulations differ in salt concentration:
• Miller: 10 g/L NaCl
• Lennox: 5 g/L NaCl
• Luria: 0.
5 g/L NaClLB Broth Base is recommended for the general maintenance and growth ofE. colias it allows for fast cell expansion and reach high cell density. Its Lennox formulation has lower levels of salts than Miller and is recommended to use with salt-sensitive antibiotics such as Zeocin, tetracycline, or chloramphenicol.
LB Broth Base formulation and preparationTo prepare 1 L of Lennox-LB media, weigh 20 g of powder, and add distilled water to 1 L. Adjust the pH value to 7.
0 with 5 N NaOH and sterilize by autoclaving for 25 minutes at 121°C. Formulation per liter: 10 g SELECT Peptone 140, 5 g SELECT Yeast Extract, and 5 g NaCl.
Primary applications
• Growth and maintenanceof E. colistrains for plasmid purification and/or protein expression
• Resuspension ofT. pyriformiscell fractionsFind the media format that fits your needsWe offer other ready-to-use media formulations, such asLB (Lennox),Terrific BrothorS.
O.
C recovery medium.
Explore other media formats forbacterial growth.
Format: Bottle
Media Type: LB Broth Base (Lennox L Broth Base)
Preparation Method: Autoclave
Quantity: 500 g
Shipping Condition: Room Temperature
Product Type: Dehydrated Culture Media
Species: Escherichia coli
Target Organism Class: Coliforms
Unit Size: Each