Thermo Fisher, 15224090, T4 DNA Ligase (1 U/μL)

4 x 500 U T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3´ hydroxyl and 5´ phosphate termini. The unique T4 DNA Ligase buffer optimizes ligation, which can be performed in 5 minutes. Single-stranded nucleic acids are not substrates for this enzyme.
ApplicationsCloning (blunt-end or cohesive-end ligation) and adding linkers or adapters to blunt-ended DNASourcePurified fromE. colilambda lysogen NM989Performance and quality testingEndodeoxyribonuclease, 3´ and 5´ exodeoxyribonuclease assays; ligation efficiency testedUnit definitionOne unit catalyzes the exchange of 1 nmol32P-labeled pyrophosphate into ATP in 20 min at 37°C. One unit is equal to approximately 300 cohesive-end ligation units.
Unit reaction conditions66 mM Tris-HCl (pH 7.
6), 6.
6 mM MgCl2, 10 mM DTT, 66 μM ATP, 3.
3 μM32P-labeled pyrophosphate, and enzyme in 0.
1 mL for 20 min at 37°C.
Compatible Buffer: 5X Reaction Buffer
Product Type: T4 DNA Ligase
Quantity: 4 x 500 U
Concentration: 1 U/μL
Enzyme: Ligase
Unit Size: Each