Thermo Fisher, 15544034, S.O.C. Medium Invitrogen™

S.
O.
C. Medium Invitrogen™ S.
O.
C. (Super Optimal broth with Catabolite repression) Medium is used in the final step of bacterial cell transformation to obtain maximal transformation efficiency ofE. coli. Catabolite repression is achieved by providing glucose in the medium and thus creating optimal metabolic conditions forE. coli. In addition, high nutrient concentration allows cells to recover after the stress induced by transformation and achieve 2–3 fold higher efficiency as compared to recovery in LB medium.
S.
O.
C. Medium formulationComposition: 2% tryptone, 0.
5% yeast extract, 10 mM NaCl, 2.
5 mM KCl, 10 mM MgCl2, 10 mM MgSO4, and 20 mM glucose.
S.
O.
C. medium has the same formulation as the rich S.
O.
B. (Super Optimal Broth) medium with additionally added 10 mM MgCl2and 20 mM glucose (as published by Hanahan in 1983).
Convenient, ready-to-use formatS.
O.
C. Medium is supplied as 10 x 10 mL bottles of ready-to-use liquid medium.
Quality testingEach lot of S.
O.
C. Medium is tested to ensure conformance with the most current approved product specifications. This currently consists of tests for pH, osmolality, sterility, and transformation efficiency using TOP10E. coli.
Find the media format that fits your needsWe offer many ready-to-use media formulations, such asLB BrothandTerrific Broth.
Explore other media formats forbacterial growth.
Format: Bottle
Media Type: S.
O.
C (Super Optimal broth with Catabolite repression)
Packaging Type: 10 mL/bottle
Preparation Method: Ready-to-Use
Quantity: 10 x 10 mL bottles
Shipping Condition: Approved for shipment at Room Temperature or on Wet or Dry Ice
Product Type: Recovery Medium
Species: Escherichia coli
Sterility: Sterile
Target Organism Class: Coliforms
Unit Size: Each