Thermo Fisher, 18054015, Uracil DNA Glycosylase

Uracil DNA Glycosylase Uracil DNA Glycosylase (uracil-N-glycosylase) removes uracil residues from the sugar moiety of single- and double-stranded DNA without destroying the phosphodiester backbone, preventing its use as a hybridization target or as a template for DNA polymerases. UDG will not remove uracil from RNA.
Applications:Helps to eliminate carryover contamination in PCR, resulting in fewer false positive results (1,2) for cloning PCR fragments (3).
Source:Purified fromE. coliexpressing theE. coli unggene on a plasmid (4,5).
Performance and Quality Testing:Exonuclease, endonuclease, and phosphatase assays.
Unit Definition:One unit catalyzes the release of 1 nmol of free uracil from3H-poly(dU) in 1 h at 37°C.
Unit Reaction Conditions:30 mM Tris-HCl (pH 8.
3), 50 mM KCl, 5 mM MgCl2, 4 μg/ml3H-poly(dU)n at 7 x 10 5 cpm/μg, and 1 unit ofenzyme in 50 μl for 10 min. at 37°C (4).
Polymerase: DNA Polymerase
Quantity: 100 units
Shipping Condition: Approved for shipment on Wet or Dry Ice
For Use With (Application): Standard PCR, Real Time PCR (qPCR)
Form: Liquid
PCR Method: qPCR
Unit Size: Each