Thermo Fisher, 18064014, SuperScript™ II Reverse Transcriptase

SuperScript™ II Reverse Transcriptase Invitrogen SuperScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increased thermal stability compared to wild-type MMLV RT. Mutations in the RNase H domain of the enzyme eliminate degradation of the RNA during first-strand cDNA synthesis, which results in higher yields of full-length cDNA.
SuperScript reverse transcriptases are the most highly trusted and widely used RTs with over 50,000 citations, reviews, and publications to date. The enzyme is purified to near homogeneity fromE. colicontaining the modified pol gene of Moloney Murine Leukemia Virus.
Features of SuperScript II Reverse Transcriptase include:
• High cDNA yields and more full-length product
• Generation of cDNA up to 12.
3 kb
• First-strand cDNA synthesis at higher temperatures than conventional MMLV RTNote:The latest member of the SuperScript RT family,SuperScript IV Reverse Transcriptase, features enhanced thermostability, processivity, yields, and performance with any RNA samples, including those of suboptimal purity or integrity.
Concentration: 200 U/μL
Final Product Type: First-Strand cDNA
Format: Stand-alone Enzyme
No. of Reactions: 50 Reactions
Optimal Reaction Temperature: 42°C
Quantity: 10,000 Units
Reaction Format: Separate components
Reagent Type: Reverse Transcription
Reverse Transcriptase: SuperScript II
Ribonuclease H Activity: Reduced
Shipping Condition: Wet Ice
Size (Final Product): Up to 12.
3 kb
Starting Material: RNA
Technique: Reverse Transcription
GC-Rich PCR Performance: High
Reaction Speed: 50 min.
Unit Size: Each