Thermo Fisher, 22600134, Opti-MEM™ Reduced Serum Medium, powder

10 L Opti-MEM™ Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction ofFetal Bovine Serumsupplementation by at least 50% with no change to growth rate or morphology. Opti-MEM™ is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM™ can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Gibco™ Opti-MEM™modificationsfor a range of cell culture applications.
This Opti-MEM™ is modified as follows:With
• L-glutamine
• Phenol RedThe complete formulation is confidential. For more information, please contactTechnical Services.
Gibco™ Opti-MEM™ is a unique media that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%.
Gibco™ Opti-MEM™ uses a sodium bicarbonate buffer system (2.
4 g/L) and therefore requires a 5-10% CO2environment to maintain physiological pH.
Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment and filtration at the time of preparation (seeprotocolfor details).
Cell Line: Sp2, AE-1, CHO, BHK-21, and HEK
Cell Type: Primary Fibroblasts
Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
Product Line: Gibco™, Opti-MEM™
Product Type: Opti-MEM I
Quantity: 10 L
Shelf Life: 30 Months From Date of Manufacture
Shipping Condition: Room Temperature
Classification: Animal Origin
Form: Powder
Sterility: Sterile-filtered
With Additives: Glutamine, Phenol Red
Unit Size: Each