Thermo Fisher, 25530031, Proteinase K, recombinant

1 g Proteinase K from the fungusEngyodontium albumis a nonspecific serine protease that is useful for general digestion of proteins.
Proteinase K remains active:
• Over a wide pH range–optimal activity between 6.
5 and 9.
5
• Under denaturing conditions–e.
g., in the presence of SDS or urea
• In the presence of metal chelating agents—e.
g., EDTA
• At comparatively high temperatures–optimum digestion temperature is 65°CApplicationsRemoval of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections forin situhybridization.
Performance and quality testingEndodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.
Unit definitionOne mAnson unit is described as that amount of enzyme that liberates 1 μmol of Folin-positive amino acid within 1 min at 37°C using hemoglobin as a substrate.
Product Type: Proteinase K
Quantity: 1 g
Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice
Concentration: >40 U/mg
Form: Powder
Unit Size: Each