Thermo Fisher, 26130, NHS-PEG4-Azide Thermo Scientific™

NHS-PEG4-Azide Thermo Scientific™ Thermo Scientific NHS-PEG4-Azide is an amine-reactive compound that can be used to derivatize primary amines of proteins or amine-coated polymer surfaces for ligation to phosphine-modified molecules.
This NHS-ester compound reacts to form covalent bonds with primary amines (e.
g., side chain of lysine residues or aminosilane-coated surfaces). The azide (N3) group reacts with phosphine-labeled molecules by a mechanism known as Staudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples.
Features of NHS-PEG4-Azide:
•Soluble—reagent easily dissolves in water-miscible solvents for subsequent dilution in aqueous reaction mixtures with cell lysates and other biological samples
•Compatible—reaction chemistry occurs effectively in simple buffer conditions; requires no accessory reagents such as copper or reducing agents
•Specific—NHS esters are specific for covalent attachment to primary amines (side chain of lysine or N-terminus of polypeptides)
•Chemoselective—azide and phosphine groups do not react or interfere with components of biological samples (bioorthogonal) but conjugate to one another with high efficiencyCell Permeability: Not determined
Description: NHS-PEG4-Azide
Form: Liquid
Labeling Method: Chemical Labeling
Molecular Weight (g/mol): 388.
37
PEGylated: Yes
Product Line: Pierce™
Quantity: 100 mg
Reactive Moiety: Amine, Azide, NHS Ester
Shipping Condition: Ambient
Solubility: DMF, DMSO
Spacer Arm Length: 18.
9 Å
Water Soluble: No
Chemical Reactivity: Amine-Phosphine
Cleavable: No
Crosslinker Type: Heterobifunctional
Format: Standard
Product Type: Crosslinker
Spacer: Medium (10 to 30 Å)
Unit Size: Each