Thermo Fisher, 36600021, McCoy's 5A (Modified) Medium, GlutaMAX™ Supplement

Quantity: 500 mL McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.
This McCoy's 5A is modified as follows:WithWithout
• High Glucose
• Sodium Pyruvate
• GlutaMAX™
• HEPES
• Bacto-peptone
• Phenol RedThe completeformulationis available.
Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. This product also includes Dr. Hsu's addition of Hanks' salts to enable use outside a CO2incubator.
Gibco™ McCoy's 5A (modified) requires serum supplementation, commonly with 10%Fetal Bovine Serum(FBS). Gibco™ McCoy's 5A (modified) uses a sodium bicarbonate buffer system (2.
2 g / L) and therefore requires a 5–10% CO2environment to maintain physiological pH.
Cell Type: Mammalian Cells
Concentration: 1 X
Culture Environment: CO2
Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
Osmolality: 280 - 320 mOsm/kg
Product Line: Gibco™, GlutaMAX™
Product Type: McCoy's 5A Medium
Quantity: 500 mL
Shelf Life: 12 Months
Shipping Condition: Room Temperature
Classification: Animal Origin-free
Culture Type: Mammalian Cell Culture
Form: Liquid
Sterility: Sterile-filtered
With Additives: High Glucose, GlutaMAX, Phenol Red, Bacto-peptone, Sodium Bicarbonate
Without Additives: No HEPES, No Sodium Pyruvate
pH: 6.
9 to 7.
1
Unit Size: Each