Thermo Fisher, 4316034, TaqMan™ MGB Probe

6,000 pmol Scale Custom TaqMan™ probes are dual-labeled primers suitable for use in any real-time PCR applicaton. They incorporate a 5' reporter dye and a 3' nonfluorescent quencher/Minor Groove Binder (MGB). Choose among FAM™, VIC™, TET™, and NED™ as reporters. Probes are HPLC-purified.
Applied Biosystems TaqMan MGB (minor groove binder) probes are dual-labeled probes used for real-time PCR applications using TaqMan chemistry. TaqMan MGB Probes incorporate a 5' fluorescent reporter dye and a 3' nonfluorescent quencher (NFQ). The NFQ offers the advantage of lower background signal, which results in better precision in quantitation. Custom TaqMan MGB probes are HPLC-purified and available with FAM, VIC, TET, and NED reporter dyes.
Shorter, more specific probesTaqMan MGB probes include a MGB moiety at the 3' end that increases the melting temperature (Tm) of the probe and stabilizes probe/target hybrids. This means that TaqMan MGB probes can be significantly shorter than traditional probes, providing better sequence discrimination and flexibility to accommodate more targets.
Nonfluorescent quencher (NFQ) maximizes sensitivityTaqMan MGB probes incorporate an NFQ to absorb (quench) signal from the fluorescent dye label at the other end of the probe. The properties of the NFQ combined with the short length of the MGB probe result in lower background signal than with non-MGB NFQ probes. Lower background means increased sensitivity and precision in your data.
Guaranteed yieldThe unit size you select is based on the minimum amount we will deliver, not on synthesis starting amount.
Approximate ship time4–6 days in North America, 4-7 days in EuropeWe also offer:Applied Biosystems custom primers and other types of TaqMan probes.
TaqMan MGB probes and primers available as predesigned and pre-formulated TaqMan Assays.
Commercial manufacturing products and services, including OEM and GMP oligo manufacturing.
Need a different dye or concentration or a larger lot size? The TaqMan Custom Assay and Oligo Service offers custom products that you can tailor to meet your experimental needs.
3'Primer Modification: MGB-NFQ
5'Primer Modification: FAM, NED, TET, VIC
For Use With (Equipment): 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
Format: Tube
Green Features: Sustainable packaging
Internal Probe Modification: None
Product Line: TaqMan™
Product Type: TaqMan Probe
Purification Method: HPLC
Purity or Quality Grade: HPLC
Quantity: 6,000 pmol Scale
Shipping Condition: Room Temperature
For Use With (Application): Real Time PCR (qPCR)
Form: Liquid
Label or Dye: FAM
Unit Size: Each