Thermo Fisher, 4352042, TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG

250 Reactions TaqMan Fast Universal Master Mix (2X), No AmpErase UNG, delivers results in 40 minutes for 40 cycles of PCR in a 20-μL reaction volume using the Applied Biosystems 7900HT and Fast 7500 Fast real-time PCR systems. The optimized formulation contains AmpliTaq Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and allowing room temperature reaction setup. Additionally, a proprietary ROX dye serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations, for superb precision on Applied Biosystems real-time PCR instruments.
ConvenienceEverything you need for TaqMan-based qPCR amplification and detection in a single-tube format. TaqMan Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR.
CompatibilityTaqMan Fast Universal PCR Master Mix (2X), No AmpErase UNG, is compatible with TaqMan Gene Expression assays.
Alternative product:TryTaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Fast Universal PCR Master Mix and added additional capabilities for your gene expression analysis.
Concentration: 2X
Fidelity (vs. Taq): 2 X
For Use With (Equipment): 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Format: Tube
No. of Reactions: 250 Reactions
Passive Reference Dye: ROX (Pre-mixed)
Polymerase: AmpliTaq DNA Polymerase
Product Line: AmpErase™, TaqMan™
Product Type: Fast Universal PCR Master Mix
Quantity: 250 reactions
Sample Type: DNA (Genomic), cDNA
Shipping Condition: Wet Ice
Sufficient For: 250 Reactions
Volume: 20 μL
Detection Method: Primer-probe
For Use With (Application): Gene Expression, Genotyping, miRNA analysis
GC-Rich PCR Performance: High
PCR Method: qPCR
Reaction Speed: Fast
Unit Size: Each