Thermo Fisher, 4385612, Fast SYBR™ Green Master Mix Applied Biosystems™

Fast SYBR™ Green Master Mix Applied Biosystems™ Fast SYBR™ Green Master Mix Real-Time PCR Master Mix Designed for Speed Obtain a fast, reliable, and cost-effective solution for your real-time PCR applications without compromising sensitivity, specificity, dynamic range, or PCR efficiency.
• Fast—Real-time PCR results in as fast as 35 minutes
• Sensitive—Detect very low copies of target
• Specific—Minimize primer-dimer and non-specific amplification
• Reproducible—Consistent amplification across a wide dynamic range Fast SYBR™ Green Master Mix contains all of the components, excluding the template and primers, in a convenient 2X master mix. It includes the following components in an optimized buffer:
• AmpliTaq™ Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and enabling reactions to be set up at room temperature
• SYBR™ Green I dye to enable detection of double-stranded DNA
• Deoxynucleotides (dNTPs) to help maintain optimal PCR results
• Uracil-DNA Glycosylase (UDG) designed to reduce carryover contamination
• Passive internal reference based on proprietary ROX™ dye to enable increased precisionAlternative productTryPowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Fast SYBR Green Master Mix and added additional capabilities for your gene expression analysis.
Concentration: 2X
For Use With (Equipment): 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Format: Vial
No. of Reactions: 500 Reactions
Passive Reference Dye: ROX (Pre-mixed)
Polymerase: AmpliTaq Fast DNA Polymerase, AmpliTaq Fast UP
Product Line: SYBR™
Product Type: Real Time PCR SYBR Master Mix
Purity or Quality Grade: UP (Ultra Pure)
Quantity: 1 x 5 mL
Sample Type: DNA (Genomic), cDNA
Shipping Condition: Dry Ice
Sufficient For: 500 Reactions of 20 μL Each
Volume: 5 mL
Detection Method: SYBR
For Use With (Application): Gene Expression
GC-Rich PCR Performance: High
Label or Dye: SYBR Green
PCR Method: qPCR
Reaction Speed: Fast
Unit Size: Each