Thermo Fisher, 4387424M, AgPath-ID™ One-Step RT-PCR Reagents with manual

500 Reactions AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.
• Recommended for RNA pathogen amplification
• Consistent amplification of RNA targets with high specificity and sensitivity
• Optimized to work with your target-specific primers and probes
• Contains ROX for quantitative fluorescent signal normalizationAgPath-ID™ One-Step RT-PCR Reagents are configured for fast and simple reaction setup. The reactions are assembled in a single tube, minimizing sample handling errors and expediting set-up time. Once assembled, results are available in approximately one hour. The 25X RT-PCR Enzyme Mix included in the kit contains highly efficient ArrayScript™ Reverse Transcriptase, a mutant MMLV RT that produces high cDNA yields, and AmpliTaq Gold™ polymerase, the preferred hot-start DNA polymerase for specific target amplification. The 2X RT-PCR Buffer has been optimized for efficient, robust reverse transcription and PCR includes the passive reference dye, ROX™ Dye, for quantitative fluorescent signal normalization.
Concentration: 25X
Description: Works on all AB platforms, but is optimized for the 7500.
Fidelity (vs. Taq): 25 X
For Use With (Equipment): 7500 System
Format: Tube
No. of Reactions: 500 Reactions
Passive Reference Dye: ROX (Pre-mixed)
Polymerase: AmpliTaq Gold DNA Polymerase
Product Line: AgPath-ID™, Ambion™
Product Type: One-Step RT-PCR Reagent
Quantity: 500 Reactions
Reverse Transcriptase: ArrayScript™
Sufficient For: 500 Reactions
Volume: 7 mL
Detection Method: Primer-probe
For Use With (Application): Animal Health, Pathogen Detection
GC-Rich PCR Performance: High
PCR Method: 1-step RT-qPCR
Reaction Speed: Standard
Unit Size: Each