Thermo Fisher, 4440887, TaqMan™ MicroRNA Assay

M (750 RT/750 PCR reactions), Made to Order Applied Biosystems TaqMan MicroRNA Assays quantitate miRNAs with the specificity and sensitivity of TaqMan Assay chemistry. A simple, two-step protocol requires only reverse transcription with an miRNA-specific primer, followed by real-time PCR with TaqMan probes.
TaqMan MicroRNA Assays are:Highly specific—quantitate only mature miRNAs, not precursorsSensitive—conserve limited samples, requires only 1–10 nanograms of total RNA or equivalentFast, simple and scalable—two-step quantitative RT-PCR assay provides high-quality results in less than three hoursOur TaqMan MicroRNA Assay catalogue is kept up to date with the Sanger database to provide comprehensive coverage with the most current annotation.
Availability and sizesMade-to-Order assays are available in four different sizes and usually ship within two weeks. Inventoried assays are prepackaged in Small size and are ready to ship. Inventoried assays may be purchased in larger sizes by ordering them as Made to Order.
SpeciesTaqMan MicroRNA Assays are available for a range of species including human, mouse, rat, Drosophila, C. elegans, and Arabidopsis. We will continue to increase the number of miRNA assays for these species in alignment with the Sanger miRBase Registry.
Assay ComponentsTaqMan MicroRNA Assays are delivered in two tubes: one containing the RT primer, the other containing the specific pre-formulated TaqMan Assay (TaqMan probe and PCR primer set).
Pre-formulated assayForward primer: 1500 nMReverse primer: 700 nMFAM-MGB probe: 200 nMRT sequences: 50 nMTaqMan miRNA Assay selection guideTaqMan MicroRNA AssaysDescription:TaqMan MicroRNA Assays employ a novel target-specific stem–loop primer during cDNA synthesis to produce a template for real-time PCRRT chemistry:miRNA-specific RTThroughput:Best for 1–10 targetsCoverage:205 species available, coverage for miRBase v.
21TaqMan Advanced miRNA AssaysDescription:TaqMan Advanced miRNA Assays employ a universal RT step for a streamlined workflow, and a universal miR-Amp step to enable highly sensitive detection by real-time PCRRT chemistry:Universal RTThroughput:Best for >10 targetsCoverage:All human, mouse, and rat miRNAs; coverage for miRBase v.
24Description: M (750 RT/750 PCR reactions), Made to Order
For Use With (Equipment): 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
Format: Tube
Green Features: Sustainable packaging
No. of Reactions: 750 Reactions
Product Line: TaqMan™
Product Type: qPCR Reagents
Quantity: M (750 RT/750 PCR reactions), Made to Order
Shipping Condition: Room Temperature
Sufficient For: 750 Reactions
GC-Rich PCR Performance: High
PCR Method: qPCR
Reaction Speed: Fast
Unit Size: Each