Thermo Fisher, 4457291, Ambion™ In Vivo GAPDH Positive Control siRNA

250 nmol Ambion™In VivosiRNA ControlsValidated siRNA controls for optimizing siRNA experiments targeting coding and noncoding transcriptsPositive Control siRNAs functionally tested in several common cell linesNegative Controls functionally proven to have minimal effects on cell proliferation and viabilityInclude Silencer™ Select modifications for enhanced specificity or Ambion™ In Vivo modifications for increased stabilityFor use in human, mouse, and rat cellsWhat are Ambion™ In Vivo siRNAs?Ambion™In VivosiRNAs are designed using the provenSilencer™ Select algorithm and incorporate chemical modifications for superior serum stability with in vivo delivery. The added serum stability does not compromise the performance of the siRNAs. Ambion™In VivosiRNAs have been shown to be non-toxic in vivo (mouse) and non-immunogenic (cell-based assays). In cell-based assays, Ambion™In VivosiRNAs exhibit equivalent or better potency thanSilencer™ Select siRNAs. The result is better confidence in your in vivo RNAi experiments, better knockdown when paired with an efficient delivery solution, and the assurance of limited non-specific effects.
GAPDH Positive Control siRNAsOur extensively validated, positive control siRNA to human, mouse, and rat GAPDH serves multiple functions. First, it is an ideal 'test' siRNA for those just beginning siRNA experiments, because it is validated to work in multiple cell lines. In addition, because it targets GAPDH mRNA, which is commonly used as an internal control, its effects are easy to assay, and thus provides an excellent tool to monitor siRNA transfection efficiency by real-time RT-PCR. The Ambion™In VivoGAPDH Positive Control siRNA shows increased serum and nuclease resistance compared withSilencer™ Select siRNAs without loss of potency or specificity and is recommended for in vivo applications.
Quality ControlAmbion™ synthesizes and purifies each Ambion™In VivosiRNA in state-of-the-art facilities to meet the highest quality standards. As part of our rigorous quality control procedures, each RNA oligonucleotide is analyzed by MALDI-TOF mass spectrometry, and analytical HPLC is used to monitor purity. To provide the utmost in quality, we also assess each annealed siRNA by gel electrophoresis to confirm that the strands anneal properly. The result is premium-quality siRNA that is purified and ready to use.
In Vivo Ready ControlsOur 'In Vivo Ready' Control siRNAs are subjected to that extra level of purification and testing required for the introduction of siRNAs into animals. After HPLC purification and annealing, each siRNA is further purified utilizing a process that removes excess salt via a semi-permeable membrane. The result is highly pure siRNA with minimal salt content, suitable for in vivo applications. In vivo siRNAs are then sterile filtered, and tested for the presence of endotoxin. At concentrations of 50 μM in presence of deionized water, in vivo Ready siRNAs contain less than 5.
0 mM Na+, less than 0.
06 mM K+, and less than 0.
02 mM Mg2+.
For Use With (Application): RNAi, Transfection
Label or Dye: Unconjugated
Product Line: Ambion™
Product Type: siRNA
Purity: HPLC
Quantity: 250 nmol
Shipping Condition: Room Temperature
Control Type: Positive Control
RNAi Type: siRNA
Target: GAPDH
Unit Size: Each