Thermo Fisher, 61100061, MEM, powder

10 x 1 L Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using themedia selector tool.
This MEM is modified as follows:WithWithout
• L-glutamine
• HEPES
• Phenol Red
• Sodium BicarbonateThe completeformulationis available.
Gibco™ MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO2incubator, or with Hanks' salts for use without CO2. This product is made with Earle’s salts.
Using MEMMEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10%Fetal Bovine Serum(FBS). MEM uses a sodium bicarbonate buffer system (2.
2 g/L) and therefore requires a 5–10% CO2environment to maintain physiological pH. Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (seeprotocolfor details).
Cell Line: HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Cell Type: Primary Rat Astrocytes
Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
Product Line: Gibco™
Product Type: MEM (Minimum Essential Medium)
Quantity: 10 x 1 L
Shelf Life: 36 Months From Date of Manufacture
Shipping Condition: Room Temperature
Classification: Animal Origin-free
Form: Powder
Serum Level: Standard Serum Supplementation
Sterility: Sterile-filtered
With Additives: Low Glucose, Glutamine, Phenol Red
Without Additives: No HEPES, No Sodium Pyruvate, No Sodium Bicarbonate
Unit Size: Each