Thermo Fisher, A11154, PichiaPink™ Expression Strain Set

PichiaPink™ Expression Strain Set The PichiaPink™ Expression Strain Set contains the 4Pichia pastorisstrains for use with the PichiaPink™ Expression System Kits. Each strain comes as a frozen stock in YPD media with 15% glycerol. All PichiaPink™ strains have theade2deletion. 3 strains have deletions of two protease genes,PEP4andPRB1. You can use the PichiaPink™ Expression Strain Set to refill your PichiaPink™ Yeast Expression System Kits.
The PichiaPink™ Expression Strain Set includes the followingPichiastrains:PichiaPink™ Strain 1:ade2PichiaPink™ Strain 2:ade2,pep4PichiaPink™ Strain 3:ade2,prb1PichiaPink™ Strain 4:ade2,prb1,pep4Select Transformants by ColorThe PichiaPink™ Yeast Expression System makes it easy to select for transformed colonies by color. Untransformed PichiaPink™ strains form pink colonies. When grown on plates without adenine, transformed PichiaPink™ strains form white colonies (Figure 1).
Get Reduced Protein DegradationThe PichiaPink™ Expression Strain Set comes with 3 strains that include knockouts in two protease genes. Protease-deficient strains reduce the need for protease inhibitors when growing your cells and improve the yield of your protein. Test a single protease knockout or the double protease knockout to find the one that works best for you.
Why Choose the PichiaPink™ Yeast Expression System?The PichiaPink™ Yeast Expression System is based on the yeastPichia pastoris. Advantages ofPichia pastorisinclude rapid growth, well-defined genetic background, simple media formulation, and easy handling. For over 30 years,Pichia pastorishas been used by labs around the world for producing hundreds of different proteins from many species including human (Ref 1, 2). The PichiaPink™ Yeast Expression System allows convenient and cost-effective protein production from small to large scales.
For information on obtaining a commercial-use license for the PichiaPink™Yeast Expression System, please inquire at outlicensing@lifetech.
com.
References1. Cereghino JL, Cregg JM. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev. 2000 Jan;24(1):45-66. [PubMed]2. Cereghino GP, Cereghino JL, Ilgen C, Cregg JM. Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Curr Opin Biotechnol. 2002 Aug;13(4):329-32. [PubMed]Bacterial or Yeast Strain: PichiaPink™
For Use With (Application): Yeast Expression
Format: Tube
High-throughput Compatibility: High-throughput Compatible
Product Type: Expression Strain Set
Quantity: 4 x 1 mL
Shipping Condition: Room Temperature
Species: P. pastoris
Unit Size: Each