Thermo Fisher, A1435102, Expi293™ Expression Medium

6 x 1 L Gibco Expi293 Expression Medium is a chemically defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density culture of Expi293F cells in the Expi293 Expression System for scalable transient protein expression.
• Supports growth of suspension 293 cultures to densities of over 1.
5 x 10^7 cells/mL
• Transfection compatible serum-free suspension culture medium enables transfection efficiencies of greater than 70% using ExpiFectamine transfection reagent
• Enables sustained, high level expression of high density transiently transfected cultures, achieving yields of up to 1 gram per liter of recombinant protein
• Supports growth and transfection of 293 cells in culture formats of less than 1 mL in multiwell plates to greater than 10L in disposable bioreactors
• Does not contain phenol redExpi293 Expression Medium is formulated with GlutaMAX-I reagent. It is ready to use, with no supplementation required. The chemically defined formulation results in high reproducibility and lot-to-lot reliability. Expi293 Expression Medium contains no human or animal-origin components. The medium is not recommended for adherent cell cultures.
Product useCustomers using Expi293 Expression Medium in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type IIDrug Master File(DMF).
Cell Line: 293E (HEK), 293 (HEK), Expi293F™, FreeStyle™ 293-F Cells, 293F
Cell Type: Embryonic Kidney Cells
Concentration: 1 X
Culture Environment: CO2
Endotoxin Level: < 1 EU/mL
Format: Bottle(s)
Included Antibiotics: No antibiotics
Inorganic Salts: Calcium, Magnesium, Sodium Phosphate
Osmolality: 275-295 mOsm/kg
Product Line: Expi293™
Product Type: Expression Medium
Purity or Quality Grade: Cell Culture Grade
Quantity: 6 x 1 L
Classification: Animal Origin-free, Chemically-defined, Protein-free, Serum-free
Culture Type: Suspension Cell Culture
Form: Liquid
Serum Level: Serum-free
Sterility: Sterile
With Additives: GlutaMAX, Sodium Pyruvate, Sodium Bicarbonate, Dextrose, Pyridoxine HCl
Without Additives: No HEPES, No Phenol Red, No Hypoxanthine, No Thymidine, No Insulin, No Vitamin A, No 2-Mercaptoethanol
Unit Size: Each