Thermo Fisher, A24229, MAX Efficiency™ Transformation Reagent for Algae

MAX Efficiency™ Transformation Reagent for Algae MAX Efficiency™ Transformation Reagent for Algae is a buffer that enhances transformation efficiency for multiple strains ofChlamydomonasspecies. One of the biggest hurdles in research and development withChlamydomonashas been the introduction of exogenous DNA intoChlamydomonasstrains due to rigid cell walls. Methods such as glass bead agitation, electroporation, and microparticle bombardment are available but often result in low transformation efficiency. MAX Efficiency™ Transformation Reagent for Algae increases the permeability of theChlamydomonascell wall and facilitates increased delivery of DNA into the cell nucleus by electroporation. Simply grow theChlamydomonascells to early log phase, harvest by centrifugation, and wash twice with MAX Efficiency™ Transformation Reagent prior to electroporation. To date, we have seen >200-fold increase in transformation efficiency over previously recommended conditions in 10 differentChlamydomonasstrains, including wild type and mutants, using circular or linear DNA, as well as PCR fragments.
• Obtain >1000 transformants/μg DNA for cell wall(+) strains
• Obtain >100 transformants/μg DNA for cell wall(-) strains
• Transform circular or linear DNA or PCR fragmentsCell Line: Chlamydomonas
Expression System: Algae
For Use With (Application): Gel Electrophoresis
High-throughput Compatibility: Not High-throughput Compatible (Manual)
No. of Reactions: 40 Reactions
Product Line: GeneArt™
Product Type: Transformation Reagent
Quantity: 40 reactions
Selection Agent (Eukaryotic): Hygromycin
Unit Size: Each