Thermo Fisher, A2494301, SILAC Advanced DMEM/F-12 Flex Media, no glucose, no phenol red

Quantity: 500 mL SILAC Advanced DMEM/F-12 Flex is Advanced DMEM/F-12 without L-arginine, L-glutamine, and L-lysine for use during SILAC protein labeling with stable isotopic labeled lysine and/or arginine. Advanced DMEM/F-12 is a widely used basal medium for supporting the growth of many different mammalian cells, which reduces the FBS requirements by 50-90% without any changes in growth rate or morphology. When supplemented with 1-2 % FBS, Advanced DMEM/F-12 Flex is capable of supporting cellular proliferation and maximum cell densities comparable to the conventional basal formulation supplemented with 5-10% FBS. Cells successfully cultured in Advanced DMEM/F-12 with no adaptation include Jurkat, WI-38, SP2, Vero, and MRC-5. We offer a variety of DMEM/F-12 modifications for a range of applications. Find the right formulation using themedia selector tool.
This Advanced DMEM/F-12 is manufactured as follows:WithWithout
• Non-essential amino acids
• Glucose
• Sodium pyruvate
• Phenol red
• L-arginine
• L-glutamine
• L-lysine
• HEPESAdvanced DMEM/F-12 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM/F-12 typically requires supplementation with 1-5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement (optional). SILAC protein labeling experiments should be conducted using Gibco™Dialyzed FBS. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced DMEM/F-12 uses a sodium bicarbonate buffer system (3.
7 g/L), and therefore requires a 5-10% CO2environment to maintain physiological pH.
cGMP Manufacturing and Quality SystemSILAC Advanced DMEM/F-12 Flex is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Cell Type: Mammalian Cells
Product Line: Gibco™
Product Type: Dulbecco's Modified Eagle Medium
Quantity: 500 mL
Shelf Life: 12 Months
Culture Type: Mammalian Cell Culture
Form: Liquid
Serum Level: Reduced Serum
With Additives: Sodium Pyruvate, Non Essential Amino Acids (NEAA)
Without Additives: No Glucose, No Glutamine, No HEPES, No Phenol Red
Unit Size: Each