Thermo Fisher, A29130, ExpiFectamine™ CHO Transfection Kit

For 10 L of culture The ExpiFectamine CHO Transfection Kit is a core component of the ExpiCHO Expression System Kit (Cat. No. A29133) and enables transient transfection of high-density cultures of Chinese hamster ovary (CHO) cells. The high-efficiency, cationic transfection reagent, coupled with an optimized transfection enhancer and culture feed, will help power the highest possible level of protein expression from ExpiCHO-S Cells (Cat. No. A29127) cultured in ExpiCHO Expression Medium (Cat. No. A2910001). All components work together synergistically with expression yields of 1–3 g per L of transfected culture demonstrated for some antibody and non-antibody proteins. Features of the ExpiFectamine CHO Transfection Kit include:Enables robust and reproducible transfection results, giving you greater confidence in your results and ability to use CHO cells from research to discoveryAchieve >90% transfection efficiencyof high-density suspension CHO cell culture, with matching transfection enhancer and culture feed to boost transfection performance and protein expressionUses one-third to one-half less plasmid DNAthan other transfection reagentsScale growth and transfectionin culture formats from <1 mL in deep well plates up to 5 L when using Nalgene angled-bottom shake flasksFlexible, reliable culture and transfection protocolswith multiple options that readily fit into current transient expression workflowsThe ExpiFectamine CHO Transfection Kit is specifically designed to work with ExpiCHO-S Cells in the ExpiCHO Expression System Kit. The transfection kit supplies the cationic ExpiFectamine CHO Transfection Reagent, ExpiFectamine CHO Transfection Enhancer, and ExpiCHO Feed. ExpiCHO-S Cells and ExpiCHO Expression Medium are available separately. Cell Line: ExpiCHO-S™, CHO-S, SFM Adapted
Classification: Animal Origin-free, Chemically-defined, Protein-free, Serum-free
Product Line: ExpiFectamine™
Product Type: CHO Transfection Kit
Quantity: For 10 L of culture
Shipping Condition: Room Temperature
Cell Type: Ovarian Cells
Format: Kit
Promoter: pcDNA™ 3.4
Transfection Technique: Lipid-based Transfection
Unit Size: Each