Thermo Fisher, A51218, Viral Production Cells 2.0

6 vials Gibco Viral Production Cells 2.
0 are a clonal cell line derived from the HEK293F parental cell line and a core component of the AAV-MAX Helper-Free AAV Production System. The cells have been adapted to the chemically definedViral Production Mediumand are maintained in suspension culture in that medium. Viral Production Cells 2.
0 are highly transfectable using theAAV-MAX Transfection Kitfor adeno-associated virus (AAV) production.
Features of the Viral Production Cells 2.
0 include:
• Clonal, 293F-derived, high-producer cell line
• Absence of SV40 large T antigen
• Optimized for high-density, suspension culture (>12 million cells/mL) in chemically defined medium
• Enable production of high AAV titers, greater than 5 x 1010vg/mL (unpurified)
• Robust scalability and passage stabilityFrozen cells are supplied in a vial containing 1 mL of cells at 1 x 107viable cells/mL in Viral Production Medium and 10% DMSO. Cells should be thawed directly into Viral Production Medium supplemented with 4 mM GlutaMAX Supplement (Cat. No. 35050038) for optimal performance.
Cell State: Frozen
Concentration: 1e7 cells/mL
For Use With (Application): AAV Production
Format: 6 vials
Media Recommendation: Gibco Viral Production Medium
No. of Cells: 1 x 107cells
Product Line: AAV-MAX
Product Type: Viral Production Cells
Quantity: 1 mL
Shipping Condition: dry ice for domestic shipment; liquid nitrogen for international shipment
Source: Human
Cell Line: Viral Production Cells 2.
0
Cell Type: Kidney (Embryonic)
Culture Type: Suspension Cell Culture
Form: Frozen
Species: Human
Unit Size: Each