Product Description
CLA IdentiFiler™ Plus PCR Amplification Kit The CLA (Cell Line Authentication) IdentiFiler Plus PCR Amplification Kit simplifies the amplification step during authentication of cell lines. It includes the same primers and allelic ladder as the widely used AmpFLSTR Identifiler Plus kit and harnesses next-generation PCR amplification technology to help provide enhanced sensitivity, a cleaner baseline, improved performance with mixtures, and the ability to overcome high levels of PCR inhibition. The kit includes GeneScan 600 LIZ Size Standard v2.
0, a five-dye-labeled, high-density size standard for the reproducible sizing of fragment analysis data.
Features of the CLA IdentiFiler Plus PCR Amplification Kit include:
• 5-dye, 16-locus STR kit compatible with all cell line databasing loci standards
• Improved version of legacy AmpFLSTR Identifiler Plus kit
• Size standard included for ease of purchase
• Compatible with SeqStudio, 3730/xL, and 3500/xL genetic analyzersCell line authentication using short tandem repeat (STR) analysis kits can be used both for human sample authentication (HSA) and mixed sample analysis (MSA). In HSA, there is a need to verify the DNA profile of the sample to check for sample cross-contamination or sample mix-up, or to perform a quality check. For MSA there is a need to deconvolute the presence of multiple contributors in a sample (chimerism) through identification of multiple DNA genotypes in the sample.
For Use With (Equipment): SeqStudio Flex™ 3500, SeqStudio™ 3730xl Genetic Analyzers
Format: Kit
Kit Type: Multicomponent
Polymerase: AmpliTaq Gold™ DNA Polymerase
Purification Target: Cell DNA
Quantity: 200 x 25 μL Reactions
Reaction Format: Tube
Sample Type: Genomic DNA
Species: Human
Starting Material: Cell Line Culture
Target Organism Class: Human cell lines
Technique: STR (Short Tandem Repeats) Analysis
Detection Method: Gel Electrophoresis
For Use With (Application): Genotyping, Cell Analysis
Form: Liquid
GC-Rich PCR Performance: High
Label or Dye: LIZ, FAM, NED, PET, VIC
PCR Method: End-point PCR
Reaction Speed: 60 min.
Unit Size: Each
Order Guidelines
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2. Please DO NOT make payment before confirmtaion.
3. Minimum order value of $1,000 USD required.
4. 100% prepayment required.
Collaboration
Tony Tang
Email: Tony.Tang@iright.com
Mobile/WhatsApp/Wechat: +86-17717886924