Thermo Fisher, A65908, CLA IdentiFiler™ Direct PCR Amplification Kit

CLA IdentiFiler™ Direct PCR Amplification Kit The CLA (Cell Line Authentication) IdentiFiler Direct PCR Amplification Kit simplifies the amplification step during authentication of cell lines. The kit enables direct amplification of the same 16 loci included in our AmpFLSTR Identifiler Direct kit, eliminating the need to perform DNA extraction or purification steps for DNA database and other single-source reference samples. Blood or buccal samples on FTA cards can be punched into PCR plates or tubes, and taken directly to PCR amplification, without reduction in data quality. Samples collected using a Buccal DNA Collector or other non-FTA substrate can be processed without heat incubation or additional workflow steps using Prep-n-Go Buffer (available separately). The CLA Identifiler kit includes GeneScan 600 LIZ Size Standard v2.
0, a five-dye-labeled, high-density size standard for the reproducible sizing of fragment analysis data.
Features of the CLA IdentiFiler Direct PCR Amplification Kit include:
• 5-dye, 16-locus STR analysis kit compatible with all cell line databasing loci standards
• Improved version of legacy AmpFLSTR Identifiler Direct kit
• Size standard included for ease of purchase
• Compatible with SeqStudio, 3730/xL, and 3500/xL genetic analyzersCell line authentication using short tandem repeat (STR) analysis kits can be used both for human sample authentication (HSA) and mixed sample analysis (MSA). In HSA, there is a need to verify the DNA profile of the sample to check for sample cross-contamination or sample mix-up, or to perform a quality check. For MSA there is a need to deconvolute the presence of multiple contributors in a sample (chimerism) through identification of multiple DNA genotypes in the sample.
For Use With (Equipment): SeqStudio Flex™ 3500, SeqStudio™ 3730xl Genetic Analyzers
Format: Kit
Isolation Technology: Direct PCR
Kit Type: Multicomponent
No. of Reactions: 200 x 25 μL Reactions
Polymerase: AmpliTaq Gold™ DNA Polymerase
Purification Target: Genomic DNA from human cells
Quantity: 200 x 25 μL Reactions
Reaction Format: Master Mix
Sample Type: Cell Line Culture
Species: Human
Target Organism Class: Cell lines
Technique: STR (Short Tandem Repeats) Analysis
Detection Method: Gel Electrophoresis
For Use With (Application): Genotyping, Cell Analysis
Form: Liquid
GC-Rich PCR Performance: High
Label or Dye: LIZ, FAM, NED, PET, VIC
PCR Method: Direct PCR
Reaction Speed: 60 min.
Unit Size: Each