Thermo Fisher, AM1350, Poly(A) Tailing Kit

Poly(A) Tailing Kit For the polyadenylation ofin vitrotranscribed RNA to enhance translation initiation efficiency. Sufficient reagents are included for 25 reactions.
• Adds a poly(A) tail of at least 150 nucleotides in length to the 3' termini of RNA
• Enhances translational efficiencyin vivo
• Reaction can be adjusted to control for tail length
• Optimized for use with RNA transcripts synthesized using the mMESSAGE mMACHINE™ KitThe Poly(A) Tailing Kit usesE. coliPoly(A) Polymerase I (E-PAP) to polyadenylate the 3'-termini ofin vitrotranscribed RNA. Polyadenylation plays an important role in the stabilization of RNA in eukaryotes and enhances the efficiency of translation initiation. In the Poly(A) Tailing Kit, theE-PAP reaction has been optimized so that mRNAs are efficiently tailed with at least 150 adenines. The additional adenine residues confer stability to the mRNA and may increase translational efficiency ofin vitrosynthesized capped RNA in microinjection and transfection experiments (see Figure).
Accessory Products:The Poly(A) Tailing Kit is optimized for use with Ambion's mMESSAGE mMACHINE™ High Yield Capped RNA Transcription Kit. mMESSAGE mMACHINE™ Kits are available with T7 (SKU #AM1334), T3 (SKU #AM1338), or SP6 (SKU #AM1330) polymerases.
Final Product Type: In Vitro Transcribed RNA (Polyadenylated)
Format: Kit
Kit Type: Tailing Kit
No. of Reactions: 25 Reactions
Product Line: Ambion™
Quantity: 25 reactions
Shipping Condition: Dry Ice
Starting Material: RNA (In Vitro Transcribed)
For Use With (Application): miRNA analysis
Unit Size: Each