Thermo Fisher, AM8045, ARCA (Anti-Reverse Cap Analog)

ARCA (Anti-Reverse Cap Analog) Ambion™ Anti-Reverse Cap Analog (ARCA) is a cap analog used duringin vitrotranscription for the generation of capped transcripts. ARCA is modified in a way that ensures incorporation in the forward orientation only. Anti-Reverse Cap Analog (ARCA) is a modified cap analog in which the 3' OH group (closer to m7G) is replaced with –OCH3(see schematic). Because of this substitution, the RNA polymerase can only initiate transcription with the remaining hydroxyl group thus forcing ARCA incorporation in the forward orientation. As a result, unlike transcripts synthesized with conventional cap analog, 100% of the transcripts synthesized with ARCA at the 5' end are translatable leading to a strong stimulatory effect on translation. Quality Control:Each Cap Analog is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity. Each is also assayed for the efficient synthesis of cappedin vitrotranscripts by using the cap analog in functional MEGAscript™In Vitrotranscription and Retic Lysate translation reactions. Accessory Products:Complete kits are available with all the necessary reagents for thein vitrotranscription of capped RNA transcripts. The mMESSAGE mMACHINE™ T7 Ultra Kit includes ARCA (SKU #AM1345). Also available are kits containing conventional cap analog; mMESSAGE mMACHINE™ T7 (SKU #AM1344), T3 (SKU #AM1348), and SP6 (SKU #AM1340) Kits. The Retic Lysate IVT™ (SKU #AM1200) forin vitrotranslation of mRNA is also available. For Use With (Application): In Vitro Transcription, synthetic mRNA, mRNA
For Use With (Equipment): mMessage mMAchine, MEGAscript
Labeling Method: RNA Labeling
Product Line: Ambion™
Product Type: Cap analog for mRNA, cap analog for synthetic mRNA
Quantity: 10 Units
Shipping Condition: Dry Ice
Concentration: 40 mM
Final Product Type: Cap Analog for In vitro Transcription, Cap Analog for mRNA
Unit Size: Each