Thermo Fisher, AM8050, Cap Analog (m7G(5')ppp(5')G), 25 A254 Units

Cap Analog (m7G(5')ppp(5')G), 25 A254 Units Ambion™ Cap Analog [m7G(5')ppp(5')G] is used for the synthesis of 5' capped RNA molecules inin vitrotranscription reactions. These molecules are translated more efficiently inin vitrotranslation systems than uncapped mRNAs. 25 A254U supplied. Substitution of cap analog for a portion of the GTP in anin vitrotranscription reaction results in the incorporation of the cap structure into a corresponding fraction of the transcripts. Capped mRNAs are generally translated more efficiently in reticulocyte lysate and wheat germin vitrotranslation systems. It is important thatin vitrotranscripts be capped for microinjection experiments because uncapped mRNAs are rapidly degraded. Cap analog is also used as a highly specific inhibitor of the initiation step of protein synthesis. Quality Control:Each Cap Analog is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity. Each is also assayed for the efficient synthesis of cappedin vitrotranscripts by using the cap analog in functional MEGAscript™In Vitrotranscription and Retic Lysate translation reactions. Accessory Products:Complete kits are available with all the necessary reagents for thein vitrotranscription of capped RNA transcripts. The mMESSAGE mMACHINE™ T7 Ultra Kit includes ARCA (SKU #AM1345). Also available are kits containing conventional cap analog; mMESSAGE mMACHINE™ T7 (SKU #AM1344), T3 (SKU #AM1348), and SP6 (SKU #AM1340) Kits. The Retic Lysate IVT™ (SKU #AM1200) forin vitrotranslation of mRNA is also available. For Use With (Application): In Vitro Transcription, synthetic mRNA, mRNA
For Use With (Equipment): mMessage mMAchine, MEGAscript
Labeling Method: RNA Labeling
Product Line: Ambion™
Product Type: Cap analog for mRNA, cap analog for synthetic mRNA
Quantity: 1 Tube
Shipping Condition: Dry Ice
Concentration: 40 mM
Final Product Type: Cap Analog for In vitro Transcription, Cap Analog for mRNA
Unit Size: Each