Thermo Fisher, B1170, BCECF, AM (2',7'-Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein, Acetoxymethyl Ester)

20 x 50 μg With a pKaof ∼6. 98, the cell-permeant, dual-excitation ratiometric pH indicatorBCECF, AMis ideal for measuring changes in the cytosolic pH of most cells. Intracellular pH measurements with BCECF are made by determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at ∼490 nm versus the emission intensity when excited at its isobestic point of ∼440 nm. Detection Method: Fluorescence
Dye Type: pH Indicator
Quantity: 20 x 50 μg
Shipping Condition: Room Temperature
For Use With (Equipment): Microplate Reader
Product Type: BCECF AM pH Indicator
Unit Size: Each