Thermo Fisher, B34950, BacLight™ Bacterial Membrane Potential Kit

BacLight™ Bacterial Membrane Potential Kit TheBacLight™ Bacterial Membrane Potential Kitprovides the fluorescent membrane-potential indicator dye, DiOC2(3), along with a proton ionophore (CCCP) and premixed buffer. DiOC2(3) at low concentrations exhibits green fluorescence in all bacterial cells, but it becomes more concentrated in healthy cells that are maintaining a membrane potential, causing the dye to self-associate and the fluorescence emission to shift to red. The red- and green-fluorescent bacterial populations are easily distinguished using a flow cytometer. CCCP is included in the kit for use as a control because it eradicates the proton gradient, eliminating bacterial membrane potential.
View additional information about allmicrobiology assays for flow cytometry.
Detection Method: Fluorescence
Dye Type: Membrane Potential Probes
Format: Tube(s)
Quantity: 1 kit
Shipping Condition: Wet Ice
Emission: 482/497
For Use With (Application): Membrane Potential Assay
For Use With (Equipment): Flow Cytometer
Product Line: BacLight
Product Type: Bacterial Membrane Potential Kit
Unit Size: Each