Thermo Fisher, C10289, Click-iT™ AHA Alexa Fluor™ 488 Protein Synthesis HCS Assay Invitrogen™

Click-iT™ AHA Alexa Fluor™ 488 Protein Synthesis HCS Assay Invitrogen™ The Click-iT® AHA Alexa Fluor® 488 Protein Synthesis HCS Assay provides a fast, sensitive, non-toxic and non-radioactive method for the detection of nascent protein synthesis utilizing fluorescence microscopy and high-content imaging. Click-iT® AHA is an amino acid analog of methionine containing an azido moiety. Similar to 35S-methionine, Click-iT® AHA is fed to cultured cells and incorporated into proteins during active protein synthesis. Detection of the incorporated amine acid utilizes a chemoselectiove ligation or “click" reaction between an azide and an alkyne, where the azido modified protein is detected with the green-fluorescent Alexa Fluor® 488 alkyne. The Click-iT® AHA Alexa Fluor® 488 Protein Synthesis HCS Assay has been successfully tested in A549 and U-2 OS cells with a variety of reagents that inhibit protein synthesis including cycloheximide, anisomycin and puromycin in both dose response and Min⁄Max format.
Detection Method: Fluorescence
Dye Type: Alexa Fluor™ 488, Hoechst 33258
Format: 96-well plate
Green Features: Less hazardous
Quantity: 2 plates
Shipping Condition: Room Temperature
Color: Blue, Green
For Use With (Application): High-content Screening (HCS)
For Use With (Equipment): Fluorescence Microscope, High Content Analysis Instrument
Product Line: Alexa Fluor, Click-iT, Molecular Probes
Product Type: HCS Assay
Unit Size: Each