Thermo Fisher, E22013, EnzChek™ Lysozyme Assay Kit

EnzChek™ Lysozyme Assay Kit The EnzChek™ Lysozyme Assay Kit provides researchers with a simple and sensitive assay to measure lysozyme activity in solution. The increase in fluorescence resulting from lysozyme activity is measured using a fluorometer or fluorescence microplate reader. See our complete line of Fluorescence Microplate assays.
• Detect lysozyme activity concentrations as low as 20 U/mL
• Use standard fluorescein (FITC) excitation/emission settings
• Format allows for continuous measurement of lysozyme activityThe assay utilizes an innovative approach to measure lysozyme activity. Micrococcus lysodeikticus cell walls are used that have been extensively labeled with fluorescein causing quench of the fluorescence signal. Active lysozyme enzyme hydrolyzes the b-(1-4)-glucosidic linkages between the N-acetylmuramic acid and N-acetyl-D-glucosamine residues in the mucopolysaccharide cell wall, relieving the quenching and yielding a dramatic increase in fluorescence that is proportional to lysozyme activity. Detection Method: Fluorescence Intensity
Quantity: 400 assays
Shipping Condition: Room Temperature
Substrate Properties: Lipid-Based Substrate
Substrate Type: Lysozyme Substrate
Target Enzyme: Lysozyme
For Use With (Application): Lysozyme Assay
For Use With (Equipment): Fluorescence Microplate Reader
Product Line: EnzChek
Product Type: Lysozyme Assay
Unit Size: Each