Thermo Fisher, E6645, EnzChek™ Pyrophosphate Assay Kit

EnzChek™ Pyrophosphate Assay Kit The EnzChek™ Pyrophosphate Assay Kit provides a rapid and highly sensitive enzymatic assay for detecting free pyrophosphate in solution. In this assay the formation of a chromophoric product is detected spectrophotometrically. See our complete line of Fluorescence Microplate assays.
• Sensitivity limit of 1 μM pyrophosphate (∼0. 2 μg/mL)
• Acommodates a pH range of 6. 5 to 8. 5The EnzChek™ Pyrophosphate Detection Kit provides a fast, convenient, and inexpensive spectrophotometric method for measuring the inorganic pyrophosphate produced by a number of biochemical reactions, such as DNA and RNA polymerizations, cyclic AMP formation by the enzyme adenylate cyclase, and the enzymatic activation of fatty acids to form their coenzyme A esters. The EnzChek™ Pyrophosphate Assay Kit is a modification of our EnzChek™ Phosphate Assay Kit (Cat. No. E6646). In the presence of inorganic phosphate, the substrate 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG) is converted by the purine nucleoside phosphorylase (PNP) enzyme to ribose 1-phosphate and 2-amino-6-merc apto-7-methylpurine. The enzymatic conversion of MESG results in a shift in absorbance maximum from 330 nm to 360 nm. The EnzChek™ Pyrophosphate Assay Kit includes the enzyme inorganic pyrophosphatase, which catalyzes conversion of pyrophosphate into two equivalents of phosphate. The phosphate is then consumed by the MESG/PNP reaction and detected by an increase in absorbance at 360 nm. Additional sensitivity is gained by the amplification of one molecule of pyrophosphate into two molecules of phosphate. Detection Method: Colorimetric
Dye Type: MESG
Format: Tube(s)
Quantity: 100 assays
Shipping Condition: Room Temperature
Color: Ultraviolet
For Use With (Application): Pyrophosphate Assay
For Use With (Equipment): Spectrophotometer
Product Line: EnzChek
Product Type: Pyrophosphate Assay
Unit Size: Each