Thermo Fisher, EN0562, Lambda Exonuclease (10 U/μL)

5,000 units Thermo Scientific Lambda Exonuclease is a highly processive 5'→3' exodeoxyribonuclease. It selectively digests the 5'-phosphorylated strand of double-stranded DNA. The enzyme exhibits low activity on single-stranded DNA and non-phosphorylated DNA, and has no activity at nicks and limited activity at gaps in DNA.
Highlights
• Active in Thermo Scientific PCR buffersApplications
• Generating single-stranded PCR products for use in:
• DNA sequencing
• Analysis of DNA single-strand conformation polymorphism (SSCP)
• Rolling circle amplification
• Producing single-stranded DNA from double-stranded DNA fragments
• Cloning of PCR productsNoteUse of this enzyme in certain applications may be covered by patents and may require a license.
Product Type: Exonuclease
Quantity: 5,000 units
Concentration: 10 U/μL
Enzyme: Exonuclease
Unit Size: Each