Thermo Fisher, EP0441, RevertAid Reverse Transcriptase (200 U/μL)

10,000 Units Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids that is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase.
Features of RevertAid Reverse Transcriptase include:
• Efficient synthesis of full-length first strand cDNA up to 13 kb
• Optimum activity at 42°C
• Active up to 50°C
• Incorporates modified nucleotides (e.
g., Cy3-, Cy5-, rhodamine-, aminoallyl-, and fluorescein-labeled nucleotides)Applications
• First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• Synthesis of cDNA for cloning and expression
• Generation of labeled cDNA probes for microarrays
• DNA labeling
• Analysis of RNA by primer extensionSourceRevertAid Reverse Transcriptase is sourced fromE.
colicells with a cloned fragment of thepolgene encoding Moloney Murine Leukemia Virus reverse transcriptase.
Concentration: 200 U/μL
Final Product Type: First-Strand cDNA
Format: Stand-alone Enzyme
Optimal Reaction Temperature: 42°C
Quantity: 10,000 Units
Reaction Format: Separate components
Reagent Type: Reverse Transcription
Reverse Transcriptase: RevertAid
Ribonuclease H Activity: Yes
Shipping Condition: Dry Ice
Size (Final Product): Up to 13 kb
Starting Material: RNA
Technique: Reverse Transcription
Reaction Speed: 60 min.
Unit Size: Each