Thermo Fisher, FD1184, FastDigest Bpu10I

Thermo Fisher, FD1184, FastDigest Bpu10I 5'CC ↓TNAGC3'3'GGANT ↑CG5'Thermo Scientific FastDigest Bpu10I restriction enzyme recognizes CCTNAGC(-5/-2)^ site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer.
Thermo Scientific FastDigest Bpu10I is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.
The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. SeeReaction Conditions for FastDigest Enzymesfor a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such asKlenow Fragment,T4 DNA Ligase, alkaline phosphatases andT4 DNA Polymeraseall have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.
For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.
Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.
Features
• 100% activity of all FastDigest enzymes in the universal buffer
• 100% buffer compatibility with downstream applications
• Complete digestion in 5–15 minutes
• Direct loading on gels
• No star activity
• 176 FastDigest enzymes availableApplications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern Blot
• Restriction fragment length polymorphism (RFLP)
• SNP analysisNote:For cleavage with FastDigest Bpu10I, at least two copies of its recognition sequence are required. DNA substrates with one site are cleaved about five times slower. Complete cleavage of some substrates by FastDigest Bpu10I is difficult to achieve.
The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.
g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended.
For methylation sensitivity, refer to product specifications.
Compatible Buffer: 10X FastDigest Buffer/FastDigest Green Buffer
Inactivation Temperature: 80°C
Overhang: 5' protruding ends
Product Type: Restriction Enzyme
Quantity: 20 μL (20 Reactions)
Enzyme: Bpu10I
Methylation Sensitivity: Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Optimal Reaction Temperature: 37°C
Product Line: FastDigest™
Research Category: Traditional Cloning
Sensitive to Heat Inactivation: Yes
Type IIS RE: No
Unit Size: Each