Thermo Fisher, K1029, CCF4-AM

20mg CCF4-AM, our LiveBLAzer™ substrate, is a lipophilic, esterified form of the CCF4 substrate which allows it to readily enter cells. Upon entry, cleavage by endogenous cytoplasmic esterases rapidly converts CCF4-AM into its negatively charged form, CCF4, which is retained in the cytosol. CCF4 is a Fluorescence Resonance Energy Transfer (FRET) substrate which consists of a cephalosporin core linking 7-hydroxycoumarin to fluorescein. This product is to be used as a FRET-based substrate for β-lactamase as a sensitive reporter of mammalian gene expression in the GeneBLAzer® technology.
Cell Permeability: Cell-Permeant
For Use With (Equipment): Flow Cytometer, Microplate Reader
Product Line: LiveBLAzer™
Quantity: 20mg
Shipping Condition: Room Temperature
Substrate: CCF4-AM
Detection Method: Fluorescence
Form: Solid
Substrate Properties: Chemical Substrate
Substrate Type: Beta-Lactamase Substrate
Unit Size: Each