Thermo Fisher, K252020, pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1R E. coli

pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1R E. coli The pCR™8/GW/TOPO™ TA Cloning Kit delivers fast, efficient and reliable cloning. Additionally, this vector is ideal for use in Gateway™ recombination cloning into various destination vectors for a multitude of applications. This pCR™8 cloning kit offers the following features:
• Fast, 5-minute TOPO™ TA Cloning delivers 95% recombinants
• 3´-T overhangs simplify cloning of Taq-amplified PCR products
• Fast growing MachI cells shorten cloning time
• Compatible with Gateway™ recombination cloning enabling access to a multitude of destination vectors
• Sequencing primer sites located close to cloning insertion site so you sequence more insert, less vector the first time
• Spectinomycin resistance gene for robust selection inE. coli
• EcoR I sites flanking the PCR product insertion site for easy excision of insertsThis kit includes One Shot™ Mach1™-T1RChemically CompetentE. coli. Cloning Method: TOPO™ with Gateway™
No. of Reactions: 20 reactions
Product Line: One Shot™
Product Type: TA Cloning Kit
Quantity: 20 Reactions
Vector: pCR
Unit Size: Each