Thermo Fisher, K531520, pLenti6.3/V5™-TOPO™ TA Cloning™ Kit

pLenti6.3/V5™-TOPO™ TA Cloning™ Kit Invitrogen's™ pLenti6. 3⁄V5™-TOPO™Cloning Kit is part of our ViraPower™ HiPerform™ Lentiviral Expression System (catalog K5310-00). The pLenti6. 3⁄V5™-TOPO™ -TA Cloning Kit contains the TOPO™- adapted ViraPower™ HiPerform™ lentiviral expression vector, pLenti6. 3⁄V5™-TOPO™ for quick PCR-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6. 3⁄V5™-TOPO™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements. Advantages
• Stable expression
• Long-term experiments
• Accurate titer of functional virus
• 5 minute, high-efficiency TOPO™ PCR cloning in three simple stepsKey Features
• HiPerform™ WPRE and cPPT elements
• CMV promoter
• V5 epitope tag at C terminus
• Blasticidin selectionKit includes
• A lacZ vector as a positive control, pLenti6. 3⁄V5-GW⁄lacZ™
• Stbl3™ competent cellsFor research use only. Not intended for any therapeutic or diagnostic use. Cell Type: Competent Cell
Cloning Method: TOPO-TA
Constitutive or Inducible System: Constitutive
Delivery Type: Lentiviral
Expression System: ViraPower™ HiPerform™ Lentiviral Expression Systems
For Use With (Application): Protein Expression
Key Functions: Viral Expression
Product Line: HiPerform™, TOPO™, ViraPower™
Product Type: Cloning Kit
Promoter: CMV
Protein Tag: V5 Epitope Tag
Quantity: 1 kit
Selection Agent (Eukaryotic): Blasticidin
Selection Marker Promoter: SV40 Promoter
Vector: pLenti, TOPO-TA Vector
Format: Kit
Unit Size: Each