Thermo Fisher, P36239, Premo Autophagy Assays with Sensor LC3B, p62, or Tandem Sensor RFP-GFP-LC3B

Red-Orange, Green, RFP-GFP-LC3B, Premo Autophagy Tandem Sensor RFP-GFP-LC3B Kit Easily monitor various stages of cellular autophagy with Premo Autophagy assays and sensors, which are based on LC3B- or p62-linked fluorescent protein chimeras, or the RFP-GFP-LC3B tandem sensor. Premo Autophagy assay kits offer the selectivity of Premo Autophagy sensors, which are based on chimeras of autophagy-associated proteins LC3B or p62, and green (GFP) and/or red fluorescent protein (RFP), with the transduction efficiency of BacMam technology. Transduction or even co-transduction can be performed in a wide variety of cells, including neurons and neural stem cells (NSCs) using a simple protocol that involves adding the BacMam 2. 0 chimeric reagent to cells, incubating overnight, and then visualizing transduction using standard GFP (green fluorescent protein) and/or RFP (red fluorescent protein) settings. Color: Red-Orange, Green
Compatible Cells: Mammalian Cells
Description: Premo Autophagy Tandem Sensor RFP-GFP-LC3B Kit
Excitation/Emission: TagRFP: 555/584EmGFP: 488/509
For Use With (Equipment): Fluorescence Microscope
Kit Contents: Autophagy Sensor RFP-GFP-LC3B, and chloroquine diphosphate
Label Type: Other Label(s) or Dye(s)
Label or Dye: RFP-GFP-LC3B
Product Line: Premo
Product Type: Autophagy Sensor
Quantity: 1 kit
Shipping Condition: Wet Ice
Storage Requirements: Store at 2°C to 6°C, protected from light. Do Not Freeze.
Detection Method: Fluorescence
Format: Tube
Unit Size: Each