Thermo Fisher, PV3177, Z'-LYTE™ Kinase Assay Kit - Ser/Thr 4 Peptide

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 4 Peptide A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon glycogen synthase, containing Ser-7 and a consensus seq Hyd-X-R-X-X-S/T-X-X, where Hyd is bulky hydrophobic, F > L > V >> A, and X is any residue. Assay: Serine/Threonine Kinase Assay
Assay Entry: Biochemical phospho-specific activity (no antibody)
Excitation/Emission: 405/465, 535 nm
For Use With (Application): FRET
Format: 384-well Plate
Label or Dye: Methylcoumarin
No. of Reactions: 800 Reactions
Quantity: 800 x 20 μL Assays
Readout: End Point
References: Biochem J. vol 296, pp. 843-9 (1993), J. Biol Chem, vol. 260, pp 14471-6 (1985)
Shipping Condition: Dry Ice
Source: Ser 7 Glycogen Synthase (KKLNRTLSVA) Based
Substrate Properties: Peptide-Based Substrate
Target Entry: MAPK8, MAPKAPK2, MAPKAPK5, CAMK2D, MAPK9, STK22B, STK22D, CAMK2A, MAPK14, MAPK10, MAPKAPK3
Target Enzyme: Serine/Threonine Kinases
Validated Kinases: MAPKAPK2, MAPKAPK3, MAPKAPK5, STK22B, STK22D, CAMK2A, CAMK2D, MAPK8, MAPK9, MAPK10, MAPK14
Product Line: Z ́-LYTE™
Unit Size: Each