Thermo Fisher, R0081, Biotin-11-dUTP Solution (1 mM)

Thermo Fisher, R0081, Biotin-11-dUTP Solution (1 mM) Thermo Scientific Biotin-11-dUTP (biotin-epsilon-aminocaproyl-[5-{3-aminoallyl}-2'-deoxyuridine-5'-triphosphate]) is supplied as 1 mM aqueous solution titrated to pH 7.
0 with NaOH and is designed for enzymatic non-radioactive labelling of DNA.
Applications
• Enzymatic non-radioactive labeling of DNA by PCR
• Nick-translation
• cDNA synthesis
• Random primed labeling
• Primer extensionBiotin-11-dUTP can be enzymatically incorporated into DNA with:
•Reverse transcriptases
•TaqDNA Polymerase
•phi29 DNA Polymerase
•Klenow Fragment, exo
•Klenow Fragment
•DNA Polymerase IConcentration: 1 mM
Labeling Method: Direct Labeling
Polymerase: DNA Polymerase
Quantity: 50 μL
Shipping Condition: Dry Ice
For Use With (Application): cDNA Synthesis, Non-Radioactive Labeling
Form: Liquid
Label or Dye: Biotin
Unit Size: Each