Thermo Fisher, SD0031, phiX174 RF1 DNA

Thermo Fisher, SD0031, phiX174 RF1 DNA Thermo Scientific phiX174 was the first DNA virus discovered to have a single-stranded, circular genome. phiX174 DNA is 5386 nt in length. The DNA strand packaged into the virion is termed the “plus” strand. After entering the cell, the Thermo Scientific phiX174 DNA is used as a template for minus-strand synthesis, producing double-stranded DNA. The conversion of plus DNA strands to double-stranded DNA does not require any of the phage genes to function.
The double-stranded DNA can then be transcribed, resulting in synthesis of phage-encoded proteins. Synthesis of single-stranded (plus) DNA requires phage-encoded gene A protein. DNA synthesis is initiated at ori (+) and proceeds in the direction indicated. Late in infection, the single-stranded circles are encapsidated into new virions. The cycle terminates by cellular lysis, mediated by phage gene E encoded protein.
Highlights
• Purified by chromatography using proprietary patented technology
• More than 90% in the supercoiled form
• Isolated from E. coli HF4704 infected with phiX174 am3Applications
• Preparation of DNA molecular weight standardsGenBank/EMBL Accession NumbersV01128, J02482, M10348, M10379, M10714, M10749, M10750, M10866, M10867, M24859phiX174 plasmid usage notes:For DNA sequence, sequence analysis and map creation, see theREviewer free online toolConcentration: 0.
5 μg/μL
Final Product Type: DNA
For Use With (Application): Cloning/Nucleic Acid Gel Electrophoresis
Gel Compatibility: Agarose Gels
Product Type: DNA Reagent
Quantity: 50 μg
Sample Type: DNA
Unit Size: 50 µg