Waters, 176004597, BioResolve SEC mAb Column with BioResolve SEC mAb Guard, 200Å, 2.5 µm, 4.6 x 300 mm, 1/pk with mAb Size Variant Standard

The BioResolve SEC mAb 4.6 x 300 mm Column and Guard contains 2.5 µm BEH particle technology and is application specifically tested with the mAb Size Variant Standard to ensure accurate and highly reproducible quantitation of monoclonal antibody (mAb) monomers from frequently associated high molecular weight aggregates (≥300,000 Da) and lower molecular weight fragments (e.g., ≤100,000 Da). This column is also available with Water mAb Size Variant Standard (p/n: 176004592) at no extra charge.

Specifications
Chemistry: SEC
Separation Mode: SEC
Particle Substrate: Hybrid
pH Range Min: 2.5 pH
pH Range Max: 8 pH
Temperature Limits: 60 C
Maximum Pressure: 6,500 psi (448 Bar)
Endcapped: No
Bonding Technology: Diol (OH)
Silanol Activity: Low
Molecular Weight Range Min: 10000
Molecular Weight Range Max: 450000
Particle Shape: Spherical
Particle Size: 2.5 µm
Endfitting Type: Parker-style
Pore Size: 200 Å
QC Tested: Protein
Format: Online Column
Packing Solvent: 10% acetonitrile/90% 25 mM sodium phosphate pH 7.0 + 100 mM KCl
Surface Area: 220
System: HPLC, UHPLC, UPLC
Particle Technology: BEH
Technique: LC
USP Classification: L33
Inner Diameter: 4.6 mm
Length: 300 mm
Carbon Load: 12 %
eCord: Yes
UNSPSC: 41115711
Application: Protein
Brand: BioResolve
Product Type: Columns
Units per Package: 1 pk

Additional Information
BioResolve SEC mAb Column with BioResolve SEC mAb Guard, 200Å, 2.5 µm, 4.6 x 300 mm, 1/pk with mAb Size Variant Standard Accurate monoclonal antibody analysis requires a keen scientist and an even keener chromatography column that can handle the separations of higher and lower molecular weight fragments. Luckily for you, we have the column part covered with the BioResolve SEC mAb Column. This monoclonal antibody analysis column offers BEH particle technology that provides incredible separation performance all the while withholding both chemical and mechanical stability. What’s more, this mAb column even comes with the accompanying BioResolve SEC mAb Guard column to protect your precious data from column contaminants. Change the way you perform mAb quantitation when you buy the BioResolve SEC mAb Column. The Ethylene-Bridged Hybrid (BEH) particle at the heart of the BioResolve SEC mAb Column enables it to perform mAb quantitation with the performance speed and selectivity that you desire. But even more, the BEH particle is responsible for withstanding extreme chromatographic conditions while upholding mechanical and chemical stability. Not only that, but thanks to the rigidity that this particle provides, we can apply a wider and more usable pH range to this column. This will especially come in handy if you’re developing methods. Speaking of method development, the BioResolve SEC mAb Column is the perfect tool for it. Why? For one, this column is LC system agnostic, which means it can work with UHPLC, HPLC and UPLC systems with the same dedication to stellar performance. What’s more, each and every BioResolve SEC mAb Column undergoes a strict QC process with the Waters Size Variant Standard before being delivered to you. This helps to ensure you only get the best performing equipment right out of the box. Ready to perform mAb quantitation? Before doing so, make sure to use the mAb Size Variant Standard on your LC system to ensure proper performance. At Waters, it’s incredibly important to us to provide products to keep your LC-MS systems operating at peak performance. That’s why we offer quality control reference materials for performance benchmarking purposes. We offer much more than just mAb columns at Waters, too. We have everything you need to be successful in the lab, including full LC-MS systems, lab informatics, sample prep solutions and so much more. Shop lab equipment to explore our offerings.

FAQ
What’s the BioResolve SEC mAb Column’s Bonding Technology? The BioResolve SEC mAb Column has Diol (OH) bonding technology.